A Liposome is a spherical vesicle with a membrane composed of a phospholipid bilayer used to deliver drug or genetic material into a cell.
Liposomes can be composed of naturally derived phospholipids with mixed lipid chains like egg phosphatidylethonalimine or of pure components like dioleoyl phosphatidylethanolamine.
Our High Pressure Homogenizer systems for Preparation of Liposomes
Our Application Specialists have undertaken multiple liposomal formulation trials. Liposomes loaded with hydrophilic and hydrophobic drugs were formulated and assessed for particle size and Polydispersity Index (PDI).
The results were very positive - as many of our customers have also discovered utilizing our technology over many years.
During these tests - using our unique technology - different processing parameters were used, including a range of different high pressures, the number of passes through our homogenizer, and using different processing heads - both piston gap valves (PG) and our micro-channel devices (MCD). Sizes from micron level to below 50 nanometers / nm can be achieved.
In most scenarios, particle sizes can be reduced, and also the narrowing of the Polydispersity Index by processing at different pressures and/or increased number of passes through the Homogenizer.
High Pressure Homogenizers - features and benefits
Provide rapid reduction of vesicle size and lamellarity to achieve smaller sizes whilst narrowing the distribution.
HPH also presents the opportunity to load the Liposomes, locating hydrophilic active substances within the aqueous vesicle core.
Ultra high pressures to 60,000 psi (4200 bar) and processing from 1 ml to 35ml in the Laboratory up to flow rates in excess of 2000 l/h.
Easy to scale - from Lab to full production systems for continuous processing with repeatable results.
Multiple options for temperature control including pre-heating and rapid post process cooling.
Piston Gap and Micro-Channel Device (interaction chamber).
We think we are unique in that we offer interchangeable piston gap (PG) and micro-channel devices (MCD) processing heads for systems. For Liposome preparation both types can be effective but we recommend (for production applications) that both technologies are tested.
Vesicle size reduction is a function of pressure, valve type (PG or MCD) and formulation. Typically sizes from micron level to below 50 nanometers / nm can be achieved.
Highly versatile, fully featured bench top instruments with pressures to 60,000 psi (410 MPa, 4200 bar).
For R&D and smaller scale production up to 125 l/h.
Full scale production systems with flow rates up to 2000 l/h offering unrivalled performance.
